| 0 | - | cover | 1 | pencils: Kristian Donaldson; inks: Kristian Donaldson; colors: Kristian Donaldson | |
| 1 | The Introduction | foreword, introduction, preface, afterword | 1 | script: Maurissa Tancharoen, Jed Whedon, Joss Whedon | |
| 2 | Captain Hammer: Be Like Me! | comic story | 8 | script: Zack Whedon; pencils: Eric Canete; inks: Eric Canete; colors: Dave Stewart; letters: Nate Piekos of Blambot | Captain Hammer; Dr. Horrible |
| 3 | Pinup by Gene Ha | cover reprint (on interior page) | 1 | pencils: Gene Ha; inks: Gene Ha; colors: Gene Ha | Captain Hammer |
| 4 | Moist: Humidity Rising | comic story | 8 | script: Zack Whedon; pencils: Farel Dalrymple; inks: Farel Dalrymple; colors: Dan Jackson; letters: Nate Piekos of Blambot | Moist (origin); Captain Hammer; Dr. Horrible |
| 5 | - | cover reprint (on interior page) | 1 | pencils: Kristian Donaldson; inks: Kristian Donaldson; colors: Kristian Donaldson | |
| 6 | Penny: Keep Your Head Up | comic story | 8 | script: Zack Whedon; pencils: Jim Rugg; inks: Jim Rugg; colors: Dan Jackson; letters: Nate Piekos of Blambot | Penny; Dr. Horrible |
| 7 | - | cover reprint (on interior page) | 1 | pencils: Kristian Donaldson; inks: Kristian Donaldson; colors: Kristian Donaldson | Dr. Horrible; Penny |
| 8 | The Evil League of Evil | comic story | 16 | script: Zack Whedon; pencils: Scott Hepburn; inks: Scott Hepburn; colors: Dan Jackson; letters: Nate Piekos of Blambot | Professor Normal; Fury Leika; Tie Die; Dead Bowie; Fake Thomas Jefferson; Bad Horse; Captain Hammer; Wingspan; Johnny Snow; James Flames |
| 9 | - | cover reprint (on interior page) | 1 | pencils: Kristian Donaldson; inks: Kristian Donaldson; colors: Kristian Donaldson | Captain Hammer; Dr. Horrible |
| 10 | Dr. Horrible | comic story | 24 | script: Zack Whedon; pencils: Joëlle Jones; inks: Joëlle Jones; colors: Dan Jackson; letters: Nate Piekos of Blambot | Dr. Horrible [Billy] (villain); Captain Hammer; Justice Joe; Bad Horse; Penny; Moist (villain); Mr. Maniacal (flashback, villain) |