| 0 | - | cover | 1 | pencils: Adam Hughes; inks: Adam Hughes; colors: Adam Hughes | Zoë Alleyne Washburne, Jayne Cobb, Malcolm Reynolds |
| 1 | - | blank page(s) | 3 | | |
| 2 | - | credits, title page | 1 | pencils: Will Conrad; inks: Will Conrad; colors: Will Conrad | Captain Malcolm "Mal" Reynolds |
| 3 | - | credits, title page | 2 | pencils: Will Conrad; inks: Will Conrad | |
| 4 | - | credits, title page | 1 | pencils: Will Conrad; inks: Will Conrad; colors: Will Conrad | Shepherd Derrial Book; Jayne Cobb; Captain Malcolm "Mal" Reynolds; Zoe Washburne |
| 5 | - | cover reprint (on interior page) | 1 | pencils: Adam Hughes; inks: Adam Hughes; colors: Adam Hughes | Malcolm Reynolds, Inara Serra |
| 6 | - | cover reprint (on interior page) | 1 | pencils: John Cassaday; inks: John Cassaday; colors: Laura Martin | Malcolm Reynolds |
| 7 | Introduction | foreword, introduction, preface, afterword | 1 | script: Nathan Fillion | |
| 8 | - | cover reprint (on interior page) | 1 | pencils: J. G. Jones; inks: J. G. Jones; colors: Laura Martin | Inara Serra |
| 9 | - | comic story | 22 | script: Joss Whedon, Brett Matthews; pencils: Will Conrad; inks: Will Conrad; colors: Laura Martin; letters: Michael Heisler | Zoë Alleyne Washburne, Shepherd Book, Jayne Cobb, Kaylee Frye, Malcolm Reynolds, Inara Serra, River Tam, Simon Tam, Wash |
| 10 | - | cover reprint (on interior page) | 1 | pencils: Bryan Hitch; inks: Bryan Hitch; colors: Laura Martin | Jayne Cobb |
| 11 | - | cover reprint (on interior page) | 1 | pencils: Jo Chen; inks: Jo Chen; colors: Jo Chen | Kaylee Frye |
| 12 | - | comic story | 22 | script: Joss Whedon, Brett Matthews; pencils: Will Conrad; inks: Will Conrad; colors: Laura Martin; letters: Michael Heisler | Zoë Alleyne Washburne, Shepherd Book, Jayne Cobb, Kaylee Frye, Malcolm Reynolds, Inara Serra, River Tam, Simon Tam, Wash |
| 13 | - | cover reprint (on interior page) | 1 | pencils: Joe Quesada; inks: Danny Miki; colors: Richard Isanove | Zoë Alleyne Washburne |
| 14 | - | cover reprint (on interior page) | 1 | pencils: Sean Phillips; inks: Sean Phillips; colors: Sean Phillips | Wash |
| 15 | - | comic story | 22 | script: Joss Whedon, Brett Matthews; pencils: Will Conrad; inks: Will Conrad; colors: Laura Martin; letters: Michael Heisler | Zoë Alleyne Washburne, Shepherd Book, Jayne Cobb, Kaylee Frye, Malcolm Reynolds, Inara Serra, River Tam, Simon Tam, Wash |
| 16 | A Brief History of the Universe, Circa 2516 A.D. | text article | 16 | script: Joss Whedon; pencils: Joshua Middleton, Leinil Francis Yu; inks: Joshua Middleton, Leinil Francis Yu; colors: Joshua Middleton, Leinil Francis Yu | River Tam; Inara Serra |
| 17 | Dark Horse | promo (ad from the publisher) | 2 | script: ? (ad copy); letters: typeset | |
| 18 | - | blank page(s) | 3 | | |
| 19 | - | promo (ad from the publisher) | 1 | script: ? (ad copy); letters: typeset | |