| 0 | Batman and Robin's Most Fantastic Foes! | cover | 1 | pencils: Dick Sprang, Sheldon Moldoff, Curt Swan; inks: Charles Paris, George Klein; letters: Ira Schnapp | |
| 1 | By Popular Demand... Batman and Robin's Most Fantastic Foes! | table of contents | 1 | letters: typeset | |
| 2 | The Parasols of Plunder | comic story | 12 | script: Bill Woolfolk; pencils: Bob Kane, Lew Sayre Schwartz; inks: Charles Paris; letters: Joe Letterese | Batman [Bruce Wayne]; Robin [Dick Grayson]; Alfred Pennyworth; Commissioner James Gordon; Mr. Rink (night watchman); unnamed prison warden; The Penguin [Oswald Cobblepot]; Lefty (villain); Duke (villain) |
| 4 | Builders of the Future! | public service announcement | 1 | script: Jack Schiff; pencils: Sheldon Moldoff; inks: Sheldon Moldoff; letters: Ira Schnapp | |
| 5 | The Fox, the Shark, and the Vulture | comic story | 12 | script: Dave Wood; pencils: Sheldon Moldoff; inks: Charles Paris | Batman [Bruce Wayne]; Robin [Dick Grayson]; Commissioner James Gordon; The Terrible Trio [The Fox; The Shark; The Vulture] (villains, introduction for all) |
| 8 | Batman; Detective Comics | promo (ad from the publisher) | 0.5 | | |
| 9 | The Ice Crimes of Mr. Zero | comic story | 8.67 | script: Dave Wood; pencils: Sheldon Moldoff; inks: Charles Paris | Batman [Bruce Wayne]; Robin [Dick Grayson]; Mr. Zero (villain, introduction and origin, later called Mr. Freeze); Marty (villain); Gus (villain); Kirk (villain); Luke (villain) |
| 10 | Mystery in Space; Ultra the Multi-Alien | promo (ad from the publisher) | 0.33 | | |
| 13 | Giant Batman | letters page | 1 | letters: typeset | |
| 14 | Catwoman's Grasshopper Chase | comic story | 8 | script: Alvin Schwartz; pencils: Jack Burnley, Fred Ray; inks: Win Mortimer; letters: Ira Schnapp | Catwoman (villain) |
| 15 | - | comic story | 0.67 | script: Henry Boltinoff; pencils: Henry Boltinoff; inks: Henry Boltinoff; letters: Henry Boltinoff | |
| 16 | - | promo (ad from the publisher) | 0.33 | | |
| 17 | The Caveman at Large | comic story | 6 | script: Bill Finger; pencils: Sheldon Moldoff; inks: Charles Paris; letters: Pat Gordon | Batman [Bruce Wayne]; Robin [Dick Grayson]; Goth [Carlin] (villain) |
| 19 | The Brave and the Bold | promo (ad from the publisher) | 0.5 | | |
| 20 | The Challenge of the Calendar Man | comic story | 12 | script: Bill Finger; pencils: Sheldon Moldoff; inks: Charles Paris | Batman [Bruce Wayne]; Robin [Dick Grayson]; Calendar Man [Julian Day; Maharajah the Magician] (villain, introduction) |
| 21 | The Joker's Utility Belt | comic story | 12 | script: David Vern; pencils: Dick Sprang; inks: Charles Paris; letters: Ira Schnapp | Batman [Bruce Wayne]; Robin [Dick Grayson]; Commissioner James Gordon; Charlie Chaplin (image); Oliver Hardy (image); Stan Laurel (image); Will Rogers (image); The Joker (villain) |
| 22 | Hey, Characters! | promo (ad from the publisher) | 1 | | |