| 0 | - | cover | 1 | pencils: Jason Pearson; inks: Jason Pearson; colors: Jason Pearson | |
| 1 | - | blank page(s) | 1 | | |
| 2 | Batman Joker's Asylum | credits, title page | 1 | | |
| 3 | Batman: Joker's Asylum | credits, title page | 2 | | |
| 4 | Editorial Credits | credits, title page | 1 | letters: typeset | |
| 5 | The Joker's Wild! | cover reprint (on interior page) | 1 | pencils: Andy Kubert; inks: Andy Kubert; colors: Edgar Delgado | Joker |
| 6 | - | illustration | 1 | pencils: Andy Kubert; inks: Andy Kubert; colors: Edgar Delgado | |
| 7 | - | comic story | 22 | script: Arvid Nelson; pencils: Alex Sanchez; inks: Alex Sanchez; colors: José Villarrubia; letters: Rob Leigh | Joker [also disguised as Gaylord Spiceland] (villain); Batman [Bruce Wayne]; Flip Felchensteiner; Condoleezza Jellyhole; Gaylord Spiceland; Marty; Barbara; Kip; Carol; Dick |
| 8 | He Who Laughs Last...! | cover reprint (on interior page) | 1 | pencils: Jason Pearson; inks: Jason Pearson; colors: Jason Pearson | Penguin [Oswald Cobblepot]; Batman [Bruce Wayne] |
| 9 | - | illustration | 1 | pencils: Jason Pearson; inks: Jason Pearson; colors: Jason Pearson | |
| 10 | - | comic story | 22 | script: Jason Aaron; pencils: Jason Pearson; inks: Jason Pearson; colors: Dave McCaig; letters: Rob Leigh | Penguin [Oswald Cobblepot] (villain); Batman [Bruce Wayne]; Allison; Lori; Violet (first appearance); Bruno (villain); Joker (host; villain) |
| 11 | Deflowered! | cover reprint (on interior page) | 1 | pencils: Guillem March; inks: Guillem March; colors: Guillem March | Poison Ivy [Pamela Isley] |
| 12 | - | illustration | 1 | pencils: Guillem March; inks: Guillem March; colors: Guillem March | |
| 13 | - | comic story | 22 | script: J. T. Krul; pencils: Guillem March; inks: Guillem March; colors: Guillem March; letters: Rob Leigh | Poison Ivy [Pamela Isley] (villain; origin); Batman [Bruce Wayne]; Commissioner James Gordon; Alfred Pennyworth; Joe Cabaret (death); Roger Hine (death); David Bennet (death); Joker (host; villain); Dr. Jason Woodrue (flashback; villain) |
| 14 | Dark Knight of the Scarecrow | cover reprint (on interior page) | 1 | pencils: Juan Doe; inks: Juan Doe; colors: Juan Doe | Scarecrow [Jonathan Crane] |
| 15 | - | illustration | 1 | pencils: Juan Doe; inks: Juan Doe; colors: Juan Doe | |
| 16 | Dark Knight of the Scarecrow | comic story | 22 | script: Joe Harris; pencils: Juan Doe; inks: Juan Doe; colors: Juan Doe; letters: Rob Leigh | Batman [Bruce Wayne]; Commissioner James Gordon; Lindsay; Heather; Bethany (death); Jack; Steve (death); Scarecrow [Jonathan Crane] (villain); Joker (villain; host) |
| 17 | Two-Face, Too! | cover reprint (on interior page) | 1 | pencils: Andy Clarke; inks: Andy Clarke; colors: Andy Clarke | Two-Face [Harvey Dent] |
| 18 | - | illustration | 1 | pencils: Andy Clarke; inks: Andy Clarke; colors: Andy Clarke | |
| 19 | - | comic story | 22 | script: David Hine; pencils: Andy Clarke; inks: Andy Clarke; colors: Andy Clarke; letters: Rob Leigh | Batman [Bruce Wayne]; Holman Hunt; Dana Hunt; Two-Face [Harvey Dent] (villain); Joker (host; villain); Mr. Castor (villain); Pollux Castor (villain) |
| 20 | - | illustration | 2 | | |
| 21 | - | promo (ad from the publisher) | 2 | letters: typeset | |
| 22 | - | blank page(s) | 1 | | |
| 23 | Everyone Is a Little Crazy. Some More Than Others. | promo (ad from the publisher) | 1 | pencils: Andy Kubert; inks: Andy Kubert | |