| 0 | Gotham's Greatest Gangsters | cover | 1 | pencils: Brian Bolland; inks: Brian Bolland; colors: Brian Bolland | The Answer, Batman, Catwoman, Jonathan Crane, Harvey Dent, Pamela Isley, Joker, Selina Kyle, Aaron Nicholson, Poison Ivy, Scarecrow, Two-Face, Bruce Wayne |
| 1 | Frontispiece | credits, title page | 1 | pencils: Dave Taylor; inks: Kevin Nowlan; colors: Tom McCraw | Scarecrow; Poison Ivy; The Riddler; Charaxes |
| 2 | Contents | table of contents | 1 | letters: typeset | |
| 3 | Scream If You Love Me | comic story | 22 | script: Alan Grant; pencils: Frank Teran; inks: Carl Critchlow; colors: Chris Chuckry; letters: Clem Robins; editing: Darren Vincenzo | Rob Frazier; Jeremiah Arkham; Sweeney; Cornelius Stirk; Joker (cameo); Two-Face (cameo); The Riddler (cameo); Ventriloquist (cameo); Scarface (cameo); Mr. Zsasz (cameo); Killer Croc; Poison Ivy (cameo); Vox (cameo); Witch (cameo); Batman (cameo); Amygdala |
| 4 | Ace of Knaves | character profile | 1 | script: Scott Beatty; pencils: Jim Aparo; inks: Dave Taylor; colors: Tom McCraw | Joker |
| 5 | The Man Who Broke the Bat | character profile | 1 | script: Chuck Dixon; pencils: Graham Nolan; inks: Sal Buscema; colors: Tom McCraw | Bane |
| 6 | Master of Fear | character profile | 1 | script: Scott Beatty; pencils: Duncan Fegredo; inks: Duncan Fegredo; colors: Tom McCraw | Scarecrow |
| 7 | The Flip Side of Evil | character profile | 1 | script: Scott Beatty; pencils: Scott McDaniel; inks: Wayne Faucher; colors: Tom McCraw | Two-Face |
| 8 | Mud Pack | character profile | 1 | script: Alan Grant; pencils: Mark Buckingham; inks: Wayne Faucher; colors: Tom McCraw | Clayfaces |
| 9 | The Killer Moth | character profile | 1 | script: Scott Beatty; pencils: Flint Henry; inks: Kevin Nowlan; colors: Tom McCraw | Charaxes |
| 10 | The Plant Mistress | character profile | 1 | script: Scott Beatty; pencils: Brian Apthorp; inks: Mark Prudeaux; colors: Tom McCraw | Poison Ivy |
| 11 | The Demon's Head | character profile | 1 | script: Scott Beatty; pencils: Brian Stelfreeze; inks: Brian Stelfreeze; colors: Brian Stelfreeze | Ra's Al Ghul |
| 12 | Lost Pages: How Bane Infiltrated Wayne Manor | comic story | 5 | script: Chuck Dixon; pencils: Eduardo Barreto; inks: Tom Palmer; colors: Noelle Giddings, Digital Chameleon | Bane; Alfred Pennyworth; Batman |
| 13 | Saving the World from Itself | character profile | 1 | script: Alan Grant; pencils: Mark Buckingham; inks: Mark Buckingham; colors: Tom McCraw | The Answer |
| 14 | Cold As Ice | character profile | 1 | script: Scott Beatty; pencils: Rodolfo Damaggio; inks: Sal Buscema; colors: Tom McCraw | Mr. Freeze |
| 15 | Nature's Female Fury | character profile | 1 | script: Chuck Dixon; pencils: Greg Land; inks: Wayne Faucher; colors: Tom McCraw | Ferak |
| 16 | Underworld Boss | character profile | 1 | script: Scott Beatty; pencils: Tom Mandrake; inks: Tom Mandrake; colors: Tom McCraw | Black Mask |
| 17 | Lost Pages: The Penguin | comic story | 2 | script: Kelley Puckett; pencils: Jim Aparo; inks: Danny Miki; colors: Noelle Giddings, Digital Chameleon; letters: John Costanza | Penguin; Mr. Freeze |
| 18 | Feature: Villain Quiz | activity | 2 | script: Devin Grayson | |
| 19 | Lost Pages: Green Lantern in Arkham | comic story | 6 | script: Ron Marz; pencils: Rodolfo Damaggio; inks: Rodolfo Damaggio; colors: Noelle Giddings, Digital Chameleon; letters: Clem Robins | Batman; Flash; Green Lantern; Joker |
| 20 | Interview: The Joker | text article | 2 | script: Kelley Puckett; pencils: Brian Bolland; inks: Brian Bolland; colors: John Higgins | Joker |
| 21 | Batman Villains Timeline | text article | 2 | script: Scott Beatty | |
| 22 | Feline Fatale | character profile | 1 | script: Scott Beatty; pencils: Jim Balent; inks: Bill Sienkiewicz; colors: Tom McCraw | Catwoman |
| 23 | House Advertisements | promo (ad from the publisher) | 2 | letters: typeset | |