| 0 | - | cover | 1 | pencils: Greg Capullo; inks: Greg Capullo; colors: FCO Plascencia | The Joker |
| 1 | - | table of contents | 1 | letters: typeset | |
| 2 | Batman #11 July 1942 | cover reprint (on interior page) | 1 | pencils: Fred Ray; inks: Jerry Robinson | Batman [Bruce Wayne]; Robin [Dick Grayson]; The Joker |
| 3 | Detective Comics #69 November 1942 | cover reprint (on interior page) | 1 | pencils: Jerry Robinson; inks: Jerry Robinson | Batman [Bruce Wayne]; Robin [Dick Grayson]; The Joker |
| 4 | Batman #251 September 1973 | cover reprint (on interior page) | 1 | pencils: Neal Adams; inks: Neal Adams; letters: Gaspar Saladino | Batman [Bruce Wayne]; The Joker |
| 5 | Scars | comic story | 10 | script: Scott Snyder; pencils: Jock; inks: Jock; colors: David Baron; letters: Tom Napolitano | Officer Gary Dell; Doctor Reeder; Bruce Wayne (flashback); The Joker |
| 6 | Detective Comics #475 February 1978 | cover reprint (on interior page) | 1 | pencils: Marshall Rogers; inks: Terry Austin; colors: Tatjana Wood; letters: John Workman | Batman [Bruce Wayne]; The Joker |
| 7 | Batman: The Killing Joke March 1988 | cover | 1 | pencils: Brian Bolland; inks: Brian Bolland; colors: Brian Bolland; letters: Richard Starkings | The Joker |
| 8 | What Comes at the End of a Joke | comic story | 10 | script: James Tynion IV; pencils: Mikel Janín; inks: Mikel Janín; colors: Jordie Bellaire; letters: Clayton Cowles | Punchline [Alexis Kaye] (origin); Dean Bob; The Joker |
| 9 | Batman #429 January 1989 | cover reprint (on interior page) | 1 | pencils: Mike Mignola; inks: Mike Mignola; colors: Anthony Tollin | The Joker |
| 10 | Kill the Batman | comic story | 6 | script: Gary Whitta, Greg Miller; pencils: Dan Mora; inks: Dan Mora; colors: Ivan Plascencia; letters: Troy Peteri | Lois Lane; Alfred Pennyworth; Commissioner James Gordon; Superman [Clark Kent; Kal-El]; Batman [Bruce Wayne] (images); Harvey Dent; The Joker; Mister Freeze [Victor Fries]; Wonder Woman [Diana of Themyscira] |
| 11 | Art by Kelley Jones & Michelle Madsen | illustration | 1 | pencils: Kelley Jones; inks: Kelley Jones; colors: Michelle Madsen | The Joker |
| 12 | Introducing the Dove Corps | comic story | 8 | script: Denny O'Neil; pencils: José Luis García-López; inks: Joe Prado; colors: Marcelo Maiolo; letters: Clem Robins | The Joker; Dove Corps |
| 13 | Art by Stjepan Šejić | illustration | 1 | pencils: Stjepan Šejić; inks: Stjepan Šejić; colors: Stjepan Šejić | The Joker |
| 14 | The War Within | comic story | 8 | script: Peter J. Tomasi; pencils: Simone Bianchi; inks: Simone Bianchi; colors: Simone Bianchi; letters: Rob Leigh | The Joker; Batman |
| 15 | Art by Ivan Reis & Marcelo Maiolo | illustration | 1 | pencils: Ivan Reis; inks: Ivan Reis; colors: Marcelo Maiolo | The Joker |
| 16 | The Last Smile | comic story | 8 | script: Paul Dini; pencils: Riley Rossmo; inks: Riley Rossmo; colors: Ivan Plascencia; letters: Deron Bennett | The Joker; Harley Quinn [Harleen Quinzel]; Poison Ivy [Pamela Isley]; Batman (in Joker's dream) |
| 17 | Art by Tim Sale & Brennan Wagner | illustration | 1 | pencils: Tim Sale; inks: Tim Sale; colors: Brennan Wagner | The Joker |
| 18 | Birthday Bugs | comic story | 8 | script: Tom Taylor; pencils: Eduardo Risso; inks: Eduardo Risso; colors: Eduardo Risso; letters: Clem Robins | The Joker; Sergio; Emiliano |
| 19 | Batman #614 June 2003 | cover reprint (on interior page) | 1 | pencils: Jim Lee; inks: Scott Williams; colors: Alex Sinclair | Batman; The Joker |
| 20 | No Heroes | comic story | 8 | script: Rafael Albuquerque, Eduardo Medeiros; pencils: Rafael Albuquerque; inks: Rafael Albuquerque; colors: Marcelo Maiolo; letters: Steve Wands | Ronald Fergunson Jr.; The Joker |
| 21 | Detective Comics #880 September 2011 | cover reprint (on interior page) | 1 | pencils: Jock; inks: Jock; colors: Jock | The Joker |
| 22 | Art by John Romita Jr., Danny Miki & Peter Steigerwald | illustration | 1 | pencils: John Romita Jr.; inks: Danny Miki; colors: Peter Steigerwald | The Joker; Harley Quinn [Harleen Quinzel] |
| 23 | Penance | comic story | 8 | script: Tony S. Daniel; pencils: Tony S. Daniel; inks: Tony S. Daniel; colors: Tomeu Morey; letters: Carlos M. Mangual | The Joker; Mr. Braccio; Earl; Father Carl |
| 24 | Art by Fiona Staples | illustration | 1 | pencils: Fiona Staples; inks: Fiona Staples; colors: Fiona Staples | The Joker |
| 25 | Two Fell into the Hornet's Nest | comic story | 8 | script: Brian Azzarello; pencils: Lee Bermejo; inks: Lee Bermejo; colors: Lee Bermejo; letters: Jared K. Fletcher | The Joker; Killer Croc; Poison Ivy; Scarecrow; Penguin; Two-Face; Riddler; Batman; Robin |
| 26 | - | credits, title page | 1 | letters: typeset | |
| 27 | - | illustration | 1 | | The Joker (playing card); Batman (shadow) |