| 0 | Joker: Last Laugh | cover | 1 | pencils: Walter Simonson; inks: Walter Simonson; colors: Tatjana Wood, Heroic Age | Joker |
| 1 | Joker: Last Laugh Secret Files and Origins | credits, title page | 1 | script: Scott Beatty; pencils: Leonard Kirk; inks: Al Williamson; letters: Kurt Hathaway | Joker |
| 2 | Contents | table of contents | 1 | letters: typeset | |
| 3 | A Clown at Midnight | comic story | 30 | script: Chuck Dixon, Scott Beatty; pencils: Pete Woods; inks: Cameron Stewart; colors: Tom McCraw, Digital Chameleon; letters: Kurt Hathaway; editing: Matt Idelson | Joker; Nightwing [Dick Grayson]; Oracle [Barbara Gordon]; Shilo Norman [Mister Miracle]; Dina Bell; Warden Zimmer; Dr. Keaton; Sgt. Misura; Mammoth [Baran Flinders] (villain); Psycho-Pirate [Roger Hayden; cameo] (villain); Mr. Mind (villain); Chiller (villain); Psimon [Simon Jones] (villain); Captai |
| 4 | Shilo Norman and Dina Bell | character profile | 1 | script: Scott Beatty; pencils: Ron Randall; inks: Al Williamson; colors: Tom McCraw, Digital Chameleon | Shilo Norman [Mister Miracle]; Dina Bell |
| 5 | Touched | comic story | 8 | script: Dan Curtis Johnson, J. H. Williams III; pencils: J. H. Williams III; inks: Mick Gray; colors: Tom McCraw, Digital Chameleon; letters: Comicraft | Cameron Chase; Agent Cliff Hansen; Oracle [Barbara Gordon]; Samantha Rollins; James Wheeler; Alan Reynolds; D.E.O. [Department of Extranormal Operations]; Harley Quinn [Harleen Quinzel] (villain); Joker (villain); Gorilla Grodd (villain) |
| 6 | Black Mass | character profile | 1 | script: Scott Beatty; pencils: Javier Saltares; inks: Keith Champagne; colors: Tom McCraw, Digital Chameleon | Black Mass [Geoffrey Thibodeux] |
| 7 | The Joker's Hard Sell | comic story | 1 | script: Scott Beatty; pencils: Leonard Kirk; inks: Al Williamson; colors: Tom McCraw, Digital Chameleon; letters: Kurt Hathaway | Joker |
| 8 | Multi-Man | character profile | 1 | script: Scott Beatty; pencils: Peter Doherty; inks: Peter Doherty; colors: Tom McCraw, Digital Chameleon | Multi-Man [Duncan Pramble] |
| 9 | Worm Food | comic story | 4 | script: Jerry Ordway; pencils: Pete Krause; inks: Dick Giordano; colors: Tom McCraw, Digital Chameleon; letters: John Costanza | Mary Marvel [Mary Batson; Mary Bromfield]; Professor Bibbowski; Francis; Mr. Mind (villain) |
| 10 | Leather | character profile | 1 | script: Chuck Dixon; pencils: Damion Scott; inks: Robert Campanella; colors: Tom McCraw, Digital Chameleon | Leather [Mary Kay Tanner] |
| 11 | Science Fun Fact of the Day! | comic story | 2 | script: Jai Nitz; pencils: Amanda Conner; inks: Jimmy Palmiotti; colors: Tom McCraw, Digital Chameleon; letters: Kurt Hathaway | Joker |
| 12 | Meathead | character profile | 1 | script: Chuck Dixon; pencils: Staz Johnson; inks: Dan Davis; colors: Tom McCraw, Digital Chameleon | Meathead [Buckley Mellon] |
| 13 | Carnivora | character profile | 1 | script: Chuck Dixon; pencils: Marcos Martín; inks: Marcos Martín; colors: Tom McCraw, Digital Chameleon | Carnivora |
| 14 | Rancor | character profile | 1 | script: Chuck Dixon; pencils: Todd Nauck; inks: Robin Riggs; colors: Tom McCraw, Digital Chameleon | Rancor [Todd Francis Oszechorski] |
| 15 | Joker's Personal Effects | character profile | 1 | script: Scott Beatty | |