| 0 | - | cover | 0 | pencils: Glenn Fabry; inks: Glenn Fabry; colors: Glenn Fabry | Reverend Jesse Custer |
| 1 | Preacher, Book One | cover | 1 | | |
| 2 | The Greatest Thing Since Sliced Bread | foreword, introduction, preface, afterword | 2 | script: Garth Ennis | |
| 3 | - | cover reprint (on interior page) | 1 | pencils: Glenn Fabry; inks: Glenn Fabry; colors: Glenn Fabry | Jesse Custer |
| 4 | The Time of the Preacher | comic story | 40 | script: Garth Ennis; pencils: Steve Dillon; inks: Steve Dillon; colors: Matt Hollingsworth; letters: Clem Robins | Cassidy (introduction); Jesse Custer (introduction); Tulip O'Hare (introduction); Hugo Root (introduction); Saint of Killers (introduction); DeBlanc; Fiore; John Wayne (cameo) |
| 5 | - | cover reprint (on interior page) | 1 | pencils: Glenn Fabry; inks: Glenn Fabry; colors: Glenn Fabry | Jesse Custer (poster); Saint of Killers |
| 6 | And Hell Followed with Him | comic story | 24 | script: Garth Ennis; pencils: Steve Dillon; inks: Steve Dillon; colors: Matt Hollingsworth; letters: Clem Robins | Arseface (introduction); Cassidy; Jesse Custer; Tulip O'Hare; Hugo Root; Saint of Killers; DeBlanc; Agent Dinnings; Fiore; Sheriff Meeker; John Wayne |
| 7 | - | cover reprint (on interior page) | 1 | pencils: Glenn Fabry; inks: Glenn Fabry; colors: Glenn Fabry | Cassidy; Jesse Custer (reflection) |
| 8 | And the Horse You Rode In On | comic story | 24 | script: Garth Ennis; pencils: Steve Dillon; inks: Steve Dillon; colors: Matt Hollingsworth; letters: Clem Robins | Arseface; Cassidy; Jesse Custer; Tulip O'Hare; Hugo Root; Saint of Killers; DeBlanc; Agent Dinnings; Fiore; Sheriff Meeker |
| 9 | - | cover reprint (on interior page) | 1 | pencils: Glenn Fabry; inks: Glenn Fabry; colors: Glenn Fabry | Jesse Custer |
| 10 | Standing Tall | comic story | 24 | script: Garth Ennis; pencils: Steve Dillon; inks: Steve Dillon; colors: Matt Hollingsworth; letters: Clem Robins | Arseface; Cassidy; Jesse Custer (also in flashback); Tulip O'Hare; Hugo Root (death) (antagonist); Saint of Killers (antagonist); DeBlanc; Agent Dinnings; Fiore; Sheriff Meeker; John Wayne (cameo) |
| 11 | - | cover reprint (on interior page) | 1 | pencils: Glenn Fabry; inks: Glenn Fabry; colors: Glenn Fabry | |
| 12 | Say a Prayer for Seven Bullets | comic story | 24 | script: Garth Ennis; pencils: Steve Dillon; inks: Steve Dillon; colors: Matt Hollingsworth; letters: Clem Robins | Cassidy; Jesse Custer; Tulip O'Hare; Si Coltrane; NYPD [Detective John Tool; Detective Paulie Bridges] |
| 13 | - | cover reprint (on interior page) | 1 | pencils: Glenn Fabry; inks: Glenn Fabry; colors: Glenn Fabry | Cassidy; Jesse Custer |
| 14 | New York's Finest | comic story | 24 | script: Garth Ennis; pencils: Steve Dillon; inks: Steve Dillon; colors: Matt Hollingsworth; letters: Clem Robins | Cassidy; Jesse Custer; Tulip O'Hare; Si Coltrane; NYPD [Detective Paulie Bridges; Lieutenant Maureen Ryeberg; Detective John Tool; Officer Venucci]; John Wayne |
| 15 | - | cover reprint (on interior page) | 1 | pencils: Glenn Fabry; inks: Glenn Fabry; colors: Glenn Fabry | Detective Paulie Bridges |
| 16 | N.Y.P.D. Blue | comic story | 24 | script: Garth Ennis; pencils: Steve Dillon; inks: Steve Dillon; colors: Matt Hollingsworth; letters: Clem Robins | Cassidy; Jesse Custer; Tulip O'Hare; Si Coltrane (death); NYPD [Detective Paulie Bridges; Lieutenant Maureen Ryeberg; Detective John Tool] |
| 17 | - | cover reprint (on interior page) | 1 | pencils: Glenn Fabry; inks: Glenn Fabry; colors: Glenn Fabry | Jesse Custer; Tulip O'Hare |
| 18 | All in the Family | comic story | 24 | script: Garth Ennis; pencils: Steve Dillon; inks: Steve Dillon; colors: Matt Hollingsworth; letters: Clem Robins | Christina Custer (flashback); Jesse Custer; John Custer (flashback, death); Jody (introduction); Marie L'Angell (introduction); Tulip O'Hare; T.C. (introduction); MacAvoy (death) |
| 19 | - | cover reprint (on interior page) | 1 | pencils: Glenn Fabry; inks: Glenn Fabry; colors: Glenn Fabry | Jesse Custer; Jody; Marie L'Angell; T.C.; Billy-Bob |
| 20 | When the Story Began | comic story | 24 | script: Garth Ennis; pencils: Steve Dillon; inks: Steve Dillon; colors: Matt Hollingsworth; letters: Clem Robins | Jesse Custer (also in flashback); John Custer (flashback); God (cameo); Jody (flashback); Christina L'Angell (flashback); Marie L'Angell (also in flashback); Tulip O'Hare; T.C. (flashback); Billy-Bob (flashback) |
| 21 | - | cover reprint (on interior page) | 1 | pencils: Glenn Fabry; inks: Glenn Fabry; colors: Glenn Fabry | Jesse Custer |
| 22 | How I Learned to Love the Lord | comic story | 24 | script: Garth Ennis; pencils: Steve Dillon; inks: Steve Dillon; colors: Matt Hollingsworth; letters: Clem Robins | Jesse Custer (also in flashback); Jody (also in flashback); Marie L'Angell (also in flashback); Tulip O'Hare (death) (also in flashback); T.C. (also in flashback); Billy-Bob (flashback, death); Lorie Bobbs (flashback) |
| 23 | - | cover reprint (on interior page) | 1 | pencils: Glenn Fabry; inks: Glenn Fabry; colors: Glenn Fabry | John Wayne |
| 24 | Pardners | comic story | 24 | script: Garth Ennis; pencils: Steve Dillon; inks: Steve Dillon; colors: Matt Hollingsworth; letters: Clem Robins | Christina Custer (flashback); Jesse Custer (also in flashback); John Custer (flashback); God; Jody; Marie L'Angell; Tulip O'Hare (also in flashback, resurrection); T.C.; John Wayne (also in flashback) |
| 25 | - | cover reprint (on interior page) | 1 | pencils: Glenn Fabry; inks: Glenn Fabry; colors: Glenn Fabry | Marie L'Angell |
| 26 | Until the End of the World | comic story | 24 | script: Garth Ennis; pencils: Steve Dillon; inks: Steve Dillon; colors: Matt Hollingsworth; letters: Clem Robins | Jesse Custer; God; Jody (death); Marie L'Angell (death); Tulip O'Hare; T.C. (death) |
| 27 | - | illustration | 1 | pencils: Tim Bradstreet; inks: Tim Bradstreet; colors: Matt Hollingsworth | Jesse Custer; Tulip |
| 28 | - | illustration | 1 | pencils: Glenn Fabry; inks: Glenn Fabry; colors: Matt Hollingsworth | Cassidy |
| 29 | - | illustration | 1 | pencils: John McCrea; inks: John McCrea; colors: Matt Hollingsworth | Saint of Killers |
| 30 | - | illustration | 1 | pencils: Doug Mahnke; inks: Doug Mahnke; colors: Matt Hollingsworth | Arseface; Sheriff Root |
| 31 | - | illustration | 1 | pencils: Joe Quesada; inks: Jimmy Palmiotti; colors: Matt Hollingsworth | Herr Starr; Cassidy; D'Aronique; the Messiah |
| 32 | - | illustration | 1 | pencils: Kieron Dwyer; inks: Kieron Dwyer; colors: Matt Hollingsworth | The Sex Detectives |
| 33 | - | illustration | 1 | pencils: Jim Lee; inks: Scott Williams; colors: Tad Ehrlich | Jesse Custer; Jodie; T.C.; Granma |
| 34 | - | illustration | 1 | pencils: Dave Gibbons; inks: Dave Gibbons; colors: Matt Hollingsworth | Jesse Custer; Cassidy |
| 35 | - | illustration | 1 | pencils: Amanda Conner; inks: Jimmy Palmiotti; colors: Matt Hollingsworth | Tulip |
| 36 | - | illustration | 1 | pencils: Carlos Ezquerra; inks: Carlos Ezquerra; colors: Matt Hollingsworth | Herr Starr; the Holy Grail |
| 37 | - | illustration | 1 | pencils: John Higgins; inks: John Higgins; colors: Matt Hollingsworth | Gran'ma L'Angelle; T.C.; Jody |
| 38 | - | illustration | 1 | pencils: Dave Johnson; inks: Dave Johnson; colors: Dave Johnson | Genesis' Parents |
| 39 | - | illustration | 1 | pencils: J. G. Jones; inks: J. G. Jones; colors: Matt Hollingsworth | The Saint of Killers |
| 40 | - | illustration | 1 | pencils: Brian Bolland; inks: Brian Bolland; colors: Matt Hollingsworth | Arseface |
| 41 | - | illustration | 1 | pencils: Bruce Timm; inks: Bruce Timm; colors: Matt Hollingsworth | Jesse Custer; Tulip; Cassidy; Herr Starr; the Saint of Killers |