| 0 | - | cover | 1 | pencils: Philip Murphy; inks: Philip Murphy; colors: Philip Murphy | Optimus Prime |
| 1 | - | credits, title page | 1 | pencils: Jack Lawrence; inks: Jack Lawrence; colors: Josh Burcham | Thundercracker; Starscream; Soundwave |
| 2 | - | credits, title page | 1 | pencils: Philip Murphy; inks: Philip Murphy; colors: Philip Murphy | Mr. Spock; Dr. Leonard McCoy [Bones]; Lt. Hikaru Sulu; Commander Kuri; Klingons |
| 3 | - | credits, title page | 1 | pencils: Derek Charm, Jack Lawrence; inks: Derek Charm, Jack Lawrence; colors: Derek Charm, Josh Burcham | Optimus Prime |
| 4 | Art by Derek Charm | cover reprint (on interior page) | 1 | pencils: Derek Charm; inks: Derek Charm; colors: Derek Charm | Dr. Leonard McCoy [Bones]; Lt. M'Ress; Captain James T. Kirk; Mr. Spock; Lt. Nyota Uhura; Optimus Prime |
| 5 | Prime's Directive Part One | comic story | 20 | script: John Barber, Mike Johnson; pencils: Philip Murphy; inks: Philip Murphy; colors: Priscilla Tramontano; letters: Christa Miesner | Captain James T. Kirk; Lt. Nyota Uhura; Mr. Spock; Lt. Arex; Lt. Hikaru Sulu; Lt. M'Ress; Optimus Prime; Megatron; Starscream; Soundwave; Airachnid; Thundercracker; Lt. Montgomery Scott [Scotty]; Dr. Leonard McCoy [Bones]; Trypticon; Commander Kuri (Klingon); unidentified Klingons; Bumblebee; Jazz; |
| 6 | Art by Paulina Ganucheau | cover reprint (on interior page) | 1 | pencils: Paulina Ganucheau; inks: Paulina Ganucheau; colors: Paulina Ganucheau | Lt. M'Ress; Ravage |
| 7 | Art by Philip Murphy | cover reprint (on interior page) | 1 | pencils: Philip Murphy; inks: Philip Murphy; colors: Philip Murphy | Captain James T. Kirk; Megatron; Optimus Prime |
| 8 | Prime's Directive Part Two | comic story | 20 | script: John Barber, Mike Johnson; pencils: Philip Murphy; inks: Philip Murphy; colors: Leonardo Ito, Josh Burcham; letters: Christa Miesner | Mr. Spock; Dr. Leonard McCoy [Bones]; Lt. Montgomery Scott [Scotty]; Optimus Prime; Captain James T. Kirk; Jazz; Bumblebee; Lt. M'Ress; Lt. Hikaru Sulu; Windblade; Arcee; Ratchet; Megatron; Airachnid; Starscream; Soundwave; Commander Kuri (Klingon); unidentified Klingons; Fortress Maximus; Trypticon |
| 9 | Art by Marcelo Ferreira Colors by Thomas Deer | cover reprint (on interior page) | 1 | pencils: Marcelo Ferreira; inks: Marcelo Ferreira; colors: Thomas Deer | Mr. Spock; Megatron; Optimus Prime |
| 10 | Art by Philip Murphy | cover reprint (on interior page) | 1 | pencils: Philip Murphy; inks: Philip Murphy; colors: Philip Murphy | Captain James T. Kirk; Lt. Nyota Uhura; Mr. Spock; Lt. Arex; Soundwave; Ravage; Rumble |
| 11 | Prime's Directive Part Three | comic story | 20 | script: John Barber, Mike Johnson; pencils: Jack Lawrence; inks: Jack Lawrence; colors: Josh Burcham; letters: Christa Miesner | Soundwave; Starscream; Airachnid; unidentified miners; Optimus Prime; Dr. Leonard McCoy [Bones]; Lt. Montgomery Scott [Scotty]; Mr. Spock; Captain James T. Kirk; Ratchet; Bumblebee; Lt. M'Ress; Lt. Hikaru Sulu; Windblade; Arcee; Jazz; Thundercracker; Commander Kuri (Klingon); Trypticon; unidentified |
| 12 | Art by Gavin Fullerton | cover reprint (on interior page) | 1 | pencils: Gavin Fullerton; inks: Gavin Fullerton; colors: Gavin Fullerton | Captain James T. Kirk; Mr. Spock; Lt. Nyota Uhura; Megatron |
| 13 | Art by Philip Murphy | cover reprint (on interior page) | 1 | pencils: Philip Murphy; inks: Philip Murphy; colors: Philip Murphy | Optimus Prime |
| 14 | Prime's Directive Part Four | comic story | 20 | script: John Barber, Mike Johnson; pencils: Philip Murphy; inks: Philip Murphy; colors: Josh Burcham; letters: Christa Miesner | Fortress Tiberius [Fortress Maximus]; Trypticon; Captain James T. Kirk; Ratchet; Jazz; Bumblebee; Lt. M'Ress; Lt. Hikaru Sulu; Dr. Leonard McCoy [Bones]; Mr. Spock; Commander Kuri (Klingon); unidentified Klingons; Megatron; Windblade; Starscream; Optimus Prime; Lt. Montgomery Scott [Scotty]; Lt. Nyo |
| 15 | Art by Priscilla Tramontano | cover reprint (on interior page) | 1 | pencils: Priscilla Tramontano; inks: Priscilla Tramontano; colors: Priscilla Tramontano | Lt. Arex; Lt. M'Ress; Captain James T. Kirk; Lt. Hikaru Sulu; Mr. Spock; Lt. Nyota Uhura; Optimus Prime; |
| 16 | Art by Philip Murphy | cover reprint (on interior page) | 1 | pencils: Philip Murphy; inks: Philip Murphy; colors: Philip Murphy | Optimus Prime; Bumblebee; Ratchet; Arcee; Fortress Tiberius [Fortress Maximus]; Megatron; Soundwave; Starscream |
| 17 | Prime's Directive Part Five | comic story | 20 | script: John Barber, Mike Johnson; pencils: Philip Murphy; inks: Philip Murphy; colors: Josh Burcham; letters: Christa Miesner | Captain James T. Kirk; Ratchet; Dr. Leonard McCoy [Bones]; Fortress Tiberius [Fortress Maximus]; Mr. Spock; Lt. Arex; Lt. Hikaru Sulu; Lt. M'Ress; Lt. Nyota Uhura; Jazz; Optimus Prime; Arcee; Bumblebee; Lt. Montgomery Scott [Scotty]; unidentified Klingons; Klingon High Council; Starscream; Megatron; |
| 18 | Art by Josh Burcham | cover reprint (on interior page) | 1 | pencils: Josh Burcham; inks: Josh Burcham; colors: Josh Burcham | |
| 19 | Art by Philip Murphy | cover reprint (on interior page) | 1 | pencils: Philip Murphy; inks: Philip Murphy; colors: Philip Murphy | Captain James T. Kirk; Mr. Spock; Dr. Leonard McCoy [Bones]; Lt. Hikaru Sulu; Lt. Arex; Optimus Prime; Bumblebee; Windblade |
| 20 | Art by Philip Murphy | cover reprint (on interior page) | 1 | pencils: Philip Murphy; inks: Philip Murphy; colors: Philip Murphy | Megatron; Starscream; Reflector; Commander Kuri; Klingons |
| 21 | Art by Derek Charm | cover reprint (on interior page) | 1 | pencils: Derek Charm; inks: Derek Charm; colors: Derek Charm | Captain James T. Kirk; Mr. Spock; Lt. Arex; Optimus Prime |
| 22 | Art by Megan Levens Colors by Charlie Kirchoff | cover reprint (on interior page) | 1 | pencils: Megan Levens; inks: Megan Levens; colors: Charlie Kirchoff | Lt. Nyota Uhura; Lt. M'Ress; Nurse Christine Chapel; Windblade; Arcee |
| 23 | Art by George Caltsoudas | cover reprint (on interior page) | 1 | pencils: George Caltsoudas; inks: George Caltsoudas; colors: George Caltsoudas | Lt. M'Ress; Bumblebee |
| 24 | Art by Chris Panda | cover reprint (on interior page) | 1 | pencils: Chris Panda; inks: Chris Panda; colors: Chris Panda | Captain James T. Kirk; Mr. Spock; Lt. M'Ress; Optimus Prime |
| 25 | - | illustration | 1 | pencils: Philip Murphy, Jack Lawrence; inks: Philip Murphy, Jack Lawrence; colors: Philip Murphy, Josh Burcham | Ratchet; Optimus Prime; Thundercracker; Megatron; Starscream; Soundwave; Commander Kuri |
| 26 | - | illustration | 1 | pencils: Philip Murphy; inks: Philip Murphy; colors: Philip Murphy | Megatron; Airachnid; Starscream; Soundwave; Commander Kuri; unidentified Klingons |