| 0 | - | cover | 1 | pencils: Steve McNiven; inks: Dexter Vines; colors: Morry Hollowell | Spider-Man; J. Jonah Jameson |
| 1 | It's a brand-new day for Spidey! | promo (ad from the publisher) | 1 | letters: typeset | |
| 2 | - | illustration | 1 | pencils: Steve McNiven | |
| 3 | - | credits, title page | 2 | pencils: Salvador Larroca; inks: Salvador Larroca; colors: Jason Keith | Spider-Man; Jackpot |
| 4 | Spider-Man: The New Status Quo! | comic story | 2 | script: Bob Gale, Marc Guggenheim, Dan Slott, Zeb Wells; pencils: John Romita Jr.; inks: Klaus Janson; colors: Dean White; letters: Virtual Calligraphy, Cory Petit | Spider-Man; Mary Jane Watson; Gwen Stacy; Harry Osborn; Aunt May Parker |
| 5 | - | cover reprint (on interior page) | 1 | pencils: Phil Jimenez; inks: Phil Jimenez | Spider-Man [Peter Parker] |
| 6 | Swing Shift | comic story | 20 | script: Dan Slott; pencils: Phil Jimenez; inks: Andy Lanning, John Dell; colors: Jeromy Cox; letters: Chris Eliopoulos of Virtual Calligraphy, Cory Petit of Virtual Calligraphy | Spider-Man [Peter Parker]; Aunt May Parker; Jackpot (first appearance); J. Jonah Jameson; Robbie Robertson; Betty Brant; Officer O'Neil; Mr. Negative (villain); Overdrive (villain); The Spot (villain); Boomerang (villain); Cobra (villain); Speed Demon (villain); Bookie (villain) |
| 7 | - | cover reprint (on interior page) | 1 | pencils: Steve McNiven; inks: Dexter Vines; colors: Morry Hollowell | Spider-Man; J. Jonah Jameson |
| 8 | Brand New Day [Part 1] | comic story | 25 | script: Dan Slott; pencils: Steve McNiven; inks: Dexter Vines; colors: Morry Hollowell; letters: Virtual Calligraphy, Cory Petit | Spider-Man [Peter Parker]; Aunt May Parker; Betty Brant; Harry Osborn; Lily Hollister; Carlie Cooper; Mia Flores; Martin Li; J. Jonah Jameson; Robbie Robertson; Mr. Negative (introduction); Spider-Man Mugger (introduction) |
| 9 | - | cover reprint (on interior page) | 1 | pencils: Steve McNiven; inks: Dexter Vines | Spider-Man |
| 10 | Crimes of the Heart | comic story | 22 | script: Dan Slott; pencils: Steve McNiven; inks: Dexter Vines; colors: Morry Hollowell; letters: VC, Cory Petit | Spider-Man [Peter Parker]; J. Jonah Jameson; Joe Robertson; Glory Grant; Betty Brant; Harry Osborn; Lily Hollister; Trina; Dooley; Sean Boyle; Marla Jameson; Bruno Karnelli; Mister Negative; Aunt May Parker |
| 11 | - | cover reprint (on interior page) | 1 | pencils: Steve McNiven; inks: Dexter Vines; colors: Morry Hollowell | |
| 12 | Blood Ties | comic story | 22 | script: Dan Slott; pencils: Steve McNiven; inks: Dexter Vines; colors: Dave Stewart; letters: Cory Petit | Spider-Man; Maggia; Harry Osborn; Lily Hollister; Carlie Cooper; Spider-Mugger [Sean Boyle]; Marla Madison; Jonah Jameson; Dexter Bennett; Joe Robertson; Mister Negative; May Parker; Vin Gonzales; Alan O'Neil |
| 13 | Park Avenue Interlude | comic story | 3 | script: Marc Guggenheim; pencils: Greg Land; inks: Jay Leisten; colors: Justin Ponsor; letters: Virtual Calligraphy, Cory Petit | Jackpot; Menace (cameo) |
| 14 | The Astonishing Aunt May! | comic story | 3 | script: Bob Gale; pencils: Phil Winslade; inks: Phil Winslade; colors: Chris Chuckry; letters: Virtual Calligraphy, Cory Petit | Aunt May |
| 15 | Harry and the Hollisters | comic story | 3 | script: Zeb Wells; pencils: Mike Deodato Jr.; inks: Mike Deodato Jr.; colors: Rain Beredo; letters: Virtual Calligraphy, Cory Petit | Harry Osborn; Lily Hollister; Bill Hollister |
| 16 | - | cover reprint (on interior page) | 1 | pencils: Salvador Larroca; inks: Salvador Larroca; colors: Stephane Peru | |
| 17 | Who's That Girl? | comic story | 22 | script: Marc Guggenheim; pencils: Salvador Larroca; inks: Salvador Larroca; colors: Jason Keith; letters: Cory Petit | Spider-Man; Dexter Bennett; Jackpot |
| 18 | Introducing Menace! | cover reprint (on interior page) | 1 | pencils: Salvador Larroca; inks: Salvador Larroca | Spider-Man; Jackpot; Menace |
| 19 | The Menace of...Menace!! | comic story | 22 | script: Marc Guggenheim; pencils: Salvador Larroca; inks: Salvador Larroca; colors: Stephane Peru; letters: Virtual Calligraphy, Cory Petit | Spider-Man; Jackpot; Blue Shield; J. Jonah Jameson; Marla Jameson; Lily Hollister; Dexter Bennett; Betty Brant; Menace |
| 20 | Manhattan Menace Mystery! | cover reprint (on interior page) | 1 | pencils: Salvador Larroca; inks: Salvador Larroca | Spider-Man; Menace |
| 21 | Lo, There Shall Come a Menace!! | comic story | 22 | script: Marc Guggenheim; pencils: Salvador Larroca; inks: Salvador Larroca; colors: Stephane Peru; letters: Virtual Calligraphy, Cory Petit | Spider-Man; Jackpot; Dexter Bennett; Sara Ehret; Menace |
| 22 | Hitch Cover | cover reprint (on interior page) | 1 | pencils: Bryan Hitch; inks: Bryan Hitch | Spider-Man |
| 23 | McNiven Sketch Variant | cover reprint (on interior page) | 1 | pencils: Steve McNiven | Spider-Man |
| 24 | - | cover reprint (on interior page) | 1 | pencils: David Finch; inks: David Finch | |
| 25 | #549 Dynamic Forces Romita variant | cover reprint (on interior page) | 1 | pencils: John Romita; inks: John Romita | Spider-Man; Menace |
| 26 | Free Comic Book Day Cover Inks | illustration | 1 | pencils: Phil Jimenez; inks: Phil Jimenez | |
| 27 | Free Comic Book Day 2007 Recap Inks | illustration | 1 | pencils: Phil Jimenez; inks: Phil Jimenez | |
| 28 | Free Comic Book Day 2007 Recap Art | illustration | 1 | pencils: Phil Jimenez; inks: Andy Lanning | |
| 29 | Corner Box Art | illustration | 1 | pencils: Steve McNiven | |
| 30 | Spider-Man Manifesto | text article | 5 | script: Tom Brevoort | |
| 31 | - | cover reprint (on interior page) | 1 | pencils: Kyle Hotz; inks: Kyle Hotz | Venom; Spider-Man; Scarlet Spider; symbiotes |
| 32 | Things Undreamt Of... | comic story | 10 | script: Dan Slott; pencils: Mark Bagley; inks: Arne Starr; colors: Joe Andreani; letters: Jim Novak | Spider-Man; Capt. Jean DeWolf; Mary Jane Parker |
| 33 | The Creators | text article | 1 | letters: typeset | |
| 34 | - | promo (ad from the publisher) | 1 | pencils: David Finch; inks: David Finch | Spider-Man; Jackpot |