| 0 | - | cover | 1 | pencils: Steve McNiven; inks: Dexter Vines; colors: Morry Hollowell | J. Jonah Jameson, Peter Parker, Spider-Man |
| 1 | - | promo (ad from the publisher) | 1 | letters: typeset | |
| 2 | - | credits, title page | 3 | letters: typeset | |
| 3 | Spider-Man: The New Status Quo! | comic story | 2 | script: Bob Gale, Marc Guggenheim, Dan Slott, Zeb Wells; pencils: John Romita Jr.; inks: Klaus Janson; colors: Dean White; letters: VC's Cory Petit | Harry Osborn, May Parker, Peter Parker, Spider-Man, Gwen Stacy, Mary Jane Watson |
| 4 | Spider-Man: Free Comic Book Day 2007 | cover reprint (on interior page) | 1 | pencils: Phil Jimenez; inks: Andy Lanning; colors: Jeromy Cox | Peter Parker, Spider-Man |
| 5 | Swing Shift | comic story | 20 | script: Dan Slott; pencils: Phil Jimenez; inks: Andy Lanning, John Dell; colors: Jeromy Cox; letters: Chris Eliopoulos, VC's Cory Petit | Quentin Beck, James Beverley, Bookie, Boomerang, Betty Brant, Cobra, Curt Connors, Max Dillon, Doctor Octopus, Sara Ehret, Electro, Vin Gonzales, Jackpot, J. Jonah Jameson |
| 6 | Amazing Spider-Man #546 | cover reprint (on interior page) | 1 | pencils: Steve McNiven; inks: Dexter Vines; colors: Morry Hollowell | J. Jonah Jameson, Peter Parker, Spider-Man |
| 7 | Brand New Day [Part 1] | comic story | 25 | script: Dan Slott; pencils: Steve McNiven; inks: Dexter Vines; colors: Morry Hollowell; letters: VC's Cory Petit | Betty Brant, Carlie Cooper, Sara Ehret, Wilson Fisk, Mac Gargan, Green Goblin, Hammerhead, Lily Hollister, Jackpot, J. Jonah Jameson, Kingpin, Martin Li, Mister Negative, Harry Osborn |
| 8 | Amazing Spider-Man #547 | cover reprint (on interior page) | 1 | pencils: Steve McNiven; inks: Dexter Vines; colors: Morry Hollowell | Peter Parker, Spider-Man |
| 9 | Crimes of the Heart | comic story | 22 | script: Dan Slott; pencils: Steve McNiven; inks: Dexter Vines; colors: Morry Hollowell; letters: VC's Cory Petit | Betty Brant, Gloria Grant, Lily Hollister, J. Jonah Jameson, Martin Li, Marla Madison Jameson, Mister Negative, Harry Osborn, May Parker, Peter Parker, Robbie Robertson, Spider-Man, Bruno Karnelli, Carmine Karnelli |
| 10 | Amazing Spider-Man #548 | cover reprint (on interior page) | 1 | pencils: Steve McNiven; inks: Dexter Vines; colors: Morry Hollowell | Peter Parker, Spider-Man |
| 11 | Blood Ties | comic story | 22 | script: Dan Slott; pencils: Steve McNiven; inks: Dexter Vines; colors: Dave Stewart; letters: VC's Cory Petit | Dexter Bennett, Carlie Cooper, Vin Gonzales, Lily Hollister, J. Jonah Jameson, Martin Li, Mister Negative, Harry Osborn, May Parker, Peter Parker, Robbie Robertson, Spider-Man, Carmine Karnelli |
| 12 | Park Avenue Interlude | comic story | 3 | script: Marc Guggenheim; pencils: Greg Land; inks: Jay Leisten; colors: Justin Ponsor; letters: VC's Cory Petit | Sara Ehret, Lily Hollister, Jackpot, Menace |
| 13 | The Astonishing Aunt May! | comic story | 3 | script: Bob Gale; pencils: Phil Winslade; inks: Phil Winslade; colors: Chris Chuckry; letters: Virtual Calligraphy, Cory Petit | May Parker |
| 14 | Harry and the Hollisters | comic story | 3 | script: Zeb Wells; pencils: Mike Deodato; inks: Mike Deodato; colors: Rainier Beredo; letters: VC's Cory Petit | Bill Hollister, Lily Hollister, Harry Osborn |
| 15 | Amazing Spider-Man #549 | cover reprint (on interior page) | 1 | pencils: Salvador Larroca; inks: Salvador Larroca; colors: Stephane Peru | Peter Parker, Spider-Man |
| 16 | Who's That Girl? | comic story | 22 | script: Marc Guggenheim; pencils: Salvador Larroca; inks: Salvador Larroca; colors: Jason Keith; letters: VC's Cory Petit | Dexter Bennett, Carlie Cooper, Sara Ehret, Lily Hollister, Jackpot, Harry Osborn, Peter Parker, Spider-Man |
| 17 | Amazing Spider-Man #550 | cover reprint (on interior page) | 1 | pencils: Salvador Larroca; inks: Salvador Larroca; colors: Jason Keith; letters: Todd Klein | Sara Ehret, Lily Hollister, Jackpot, Menace, Peter Parker, Spider-Man |
| 18 | The Menace of...Menace!! | comic story | 22 | script: Marc Guggenheim; pencils: Salvador Larroca; inks: Salvador Larroca; colors: Stephane Peru; letters: VC's Cory Petit | Dexter Bennett, Blue Shield, Betty Brant, Joey Cartelli, Sara Ehret, Lily Hollister, Jackpot, J. Jonah Jameson, Marla Madison Jameson, Menace, Peter Parker, Spider-Man |
| 19 | Amazing Spider-Man #551 | cover reprint (on interior page) | 1 | pencils: Salvador Larroca; inks: Salvador Larroca; colors: Jason Keith; letters: Todd Klein | Lily Hollister, Menace, Peter Parker, Spider-Man |
| 20 | Lo, There Shall Come a Menace!! | comic story | 22 | script: Marc Guggenheim; pencils: Salvador Larroca; inks: Salvador Larroca; colors: Stephane Peru; letters: VC's Cory Petit | Dexter Bennett, Sara Ehret, Lily Hollister, Jackpot, Menace, Peter Parker, Spider-Man |
| 21 | Amazing Spider-Man #546 Variant | cover reprint (on interior page) | 1 | pencils: Bryan Hitch; inks: Bryan Hitch; colors: Laura Martin | Peter Parker, Spider-Man |
| 22 | Amazing Spider-Man #546 Sketch Variant | cover reprint (on interior page) | 1 | pencils: Steve McNiven | Peter Parker, Spider-Man |
| 23 | Amazing Spider-Man #549 Variant | cover reprint (on interior page) | 1 | pencils: David Finch; inks: David Finch; colors: Laura Martin | Sara Ehret, Jackpot, Peter Parker, Spider-Man |
| 24 | Amazing Spider-Man #549 Dynamic Forces Variant | cover reprint (on interior page) | 1 | pencils: John Romita; inks: John Romita | Lily Hollister, Menace, Peter Parker, Spider-Man |
| 25 | Spider-Man: Free Comic Book Day 2007 Cover Inks | cover reprint (on interior page) | 1 | pencils: Phil Jimenez; inks: Andy Lanning | Peter Parker, Spider-Man |
| 26 | Spider-Man: Free Comic Book Day 2007 Recap Inks | illustration | 1 | pencils: Phil Jimenez | |
| 27 | Spider-Man: Free Comic Book Day 2007 Recap Art | illustration | 1 | pencils: Phil Jimenez | |
| 28 | Corner Box Art | illustration | 1 | pencils: Steve McNiven | |
| 29 | Spider-Man Manifesto | text article | 5 | script: Tom Brevoort | |
| 30 | Planet of the Symbiotes - Part 3 of Five | cover reprint (on interior page) | 1 | pencils: Kyle Hotz; inks: Kyle Hotz | Eddie Brock, Peter Parker, Ben Reilly, Scarlet Spider, Spider-Man, Venom |
| 31 | Things Undreamt Of... | comic story | 10 | script: Dan Slott; pencils: Mark Bagley; inks: Arne Starr; colors: Joe Andreani; letters: Jim Novak | Black Cat, Jean DeWolff, Felicia Hardy, Peter Parker, Spider-Man, Mary Jane Watson |
| 32 | - | promo (ad from the publisher) | 1 | letters: typeset | |
| 33 | - | promo (ad from the publisher) | 1 | pencils: David Finch; inks: David Finch; colors: Laura Martin | Sara Ehret, Jackpot, Peter Parker, Spider-Man |