| 0 | - | cover | 1 | pencils: Phil Jimenez; inks: Andy Lanning; colors: Jeromy Cox | Peter Parker, Spider-Man |
| 1 | Title Page and Credits | credits, title page | 3 | pencils: Salvador Larroca; inks: Salvador Larroca; colors: Stephane Peru | |
| 2 | Spider-Man: The New Status Quo! | comic story | 2 | script: Bob Gale, Marc Guggenheim, Dan Slott, Zeb Wells; pencils: John Romita Jr.; inks: Klaus Janson; colors: Dean White; letters: VC's Cory Petit | Harry Osborn, May Parker, Peter Parker, Spider-Man, Gwen Stacy, Mary Jane Watson |
| 3 | - | cover reprint (on interior page) | 1 | pencils: Phil Jimenez; inks: Andy Lanning; colors: Jeromy Cox | Peter Parker, Spider-Man |
| 4 | Swing Shift | comic story | 21 | script: Dan Slott; pencils: Phil Jimenez; inks: Andy Lanning, John Dell; colors: Jeromy Cox; letters: Chris Eliopoulos of Virtual Calligraphy, VC's Cory Petit | Quentin Beck, James Beverley, Boomerang, Betty Brant, Cobra, Curt Connors, Max Dillon, Doctor Octopus, Electro, Vin Gonzales, J. Jonah Jameson, Martin Li, Lizard, Flint Marko |
| 5 | - | cover reprint (on interior page) | 1 | pencils: Steve McNiven; inks: Dexter Vines; colors: Morry Hollowell | J. Jonah Jameson, Peter Parker, Spider-Man |
| 6 | Brand New Day [Part 1] | comic story | 25 | script: Dan Slott; pencils: Steve McNiven; inks: Dexter Vines; colors: Morry Hollowell; letters: VC's Cory Petit | Betty Brant, Carlie Cooper, Wilson Fisk, Mac Gargan, Green Goblin, Hammerhead, Lily Hollister, J. Jonah Jameson, Kingpin, Martin Li, Mister Negative, Harry Osborn, Norman Osborn, May Parker |
| 7 | - | cover reprint (on interior page) | 1 | pencils: Steve McNiven; inks: Dexter Vines; colors: Morry Hollowell | Peter Parker, Spider-Man |
| 8 | Crimes of the Heart | comic story | 22 | script: Dan Slott; pencils: Steve McNiven; inks: Dexter Vines; colors: Morry Hollowell; letters: VC's Cory Petit | Betty Brant, Gloria Grant, Lily Hollister, J. Jonah Jameson, Martin Li, Marla Madison Jameson, Mister Negative, Harry Osborn, May Parker, Peter Parker, Robbie Robertson, Spider-Man, Bruno Karnelli, Carmine Karnelli |
| 9 | - | cover reprint (on interior page) | 1 | pencils: Steve McNiven; inks: Dexter Vines; colors: Morry Hollowell | Peter Parker, Spider-Man |
| 10 | Blood Ties | comic story | 22 | script: Dan Slott; pencils: Steve McNiven; inks: Dexter Vines; colors: Dave Stewart; letters: VC's Cory Petit | Dexter Bennett, Carlie Cooper, Vin Gonzales, Lily Hollister, J. Jonah Jameson, Martin Li, Marla Madison Jameson, Mister Negative, Harry Osborn, May Parker, Peter Parker, Robbie Robertson, Spider-Man, Carmine Karnelli |
| 11 | Park Avenue Interlude | comic story | 3 | script: Marc Guggenheim; pencils: Greg Land; inks: Jay Leisten; colors: Justin Ponsor; letters: VC's Cory Petit | Lily Hollister, Menace |
| 12 | The Astonishing Aunt May! | comic story | 3 | script: Bob Gale; pencils: Phil Winslade; inks: Phil Winslade; colors: Chris Chuckry; letters: VC's Cory Petit | May Parker |
| 13 | Harry and the Hollisters | comic story | 3 | script: Zeb Wells; pencils: Mike Deodato; inks: Mike Deodato; colors: Rain Beredo; letters: VC's Cory Petit | Bill Hollister, Lily Hollister, Harry Osborn |
| 14 | - | cover reprint (on interior page) | 1 | pencils: Salvador Larroca; inks: Salvador Larroca; colors: Stephane Peru | Peter Parker, Spider-Man |
| 15 | Who's That Girl? | comic story | 22 | script: Marc Guggenheim; pencils: Salvador Larroca; inks: Salvador Larroca; colors: Jason Keith; letters: VC's Cory Petit | Dexter Bennett, Carlie Cooper, Lily Hollister, Harry Osborn, Peter Parker, Spider-Man |
| 16 | Introducing Menace! | cover reprint (on interior page) | 1 | pencils: Salvador Larroca; inks: Salvador Larroca; colors: Jason Keith; letters: Todd Klein | Lily Hollister, Menace, Peter Parker, Spider-Man |
| 17 | The Menace of...Menace!! | comic story | 22 | script: Marc Guggenheim; pencils: Salvador Larroca; inks: Salvador Larroca; colors: Stephane Peru; letters: VC's Cory Petit | Dexter Bennett, Betty Brant, Lily Hollister, J. Jonah Jameson, Marla Madison Jameson, Menace, Peter Parker, Spider-Man |
| 18 | Manhattan Menace Mystery! | cover reprint (on interior page) | 1 | pencils: Salvador Larroca; inks: Salvador Larroca; colors: Jason Keith; letters: Todd Klein | Lily Hollister, Menace, Peter Parker, Spider-Man |
| 19 | Lo, There Shall Come a Menace!! | comic story | 22 | script: Marc Guggenheim; pencils: Salvador Larroca; inks: Salvador Larroca; colors: Stephane Peru; letters: VC's Cory Petit | Dexter Bennett, Lily Hollister, Menace, Peter Parker, Spider-Man |
| 20 | - | cover reprint (on interior page) | 1 | pencils: Phil Jimenez; inks: Andy Lanning; colors: Jeromy Cox | Peter Parker, Spider-Man |
| 21 | Just Blame Spider-Man | comic story | 23 | script: Bob Gale; pencils: Phil Jimenez; inks: Andy Lanning; colors: Jeromy Cox; letters: VC's Cory Petit | Frances Barrison, Dexter Bennett, Black Mamba, Bookie, Betty Brant, Bulldozer, Carlie Cooper, Eliot Franklin, Vin Gonzales, Bill Hollister, Lily Hollister, J. Jonah Jameson, John Ladue, Edward Lansky |
| 22 | - | cover reprint (on interior page) | 1 | pencils: Phil Jimenez; inks: Phil Jimenez; colors: Jeromy Cox | Peter Parker, Spider-Man |
| 23 | Freak-Out! | comic story | 22 | script: Bob Gale; pencils: Phil Jimenez; inks: Andy Lanning; colors: Jeromy Cox; letters: VC's Joe Caramagna | Dexter Bennett, Bookie, Bulldozer, Curt Connors, Carlie Cooper, Randall Crowne, Vin Gonzales, Bill Hollister, Lily Hollister, J. Jonah Jameson, John Ladue, May Parker, Peter Parker, Peter Petruski |
| 24 | The Fiery Fury of Freak! | cover reprint (on interior page) | 1 | pencils: Phil Jimenez; inks: Phil Jimenez; colors: Jeromy Cox; letters: Todd Klein | Peter Parker, Spider-Man |
| 25 | Burned! | comic story | 23 | script: Bob Gale; pencils: Phil Jimenez; inks: Andy Lanning, Danny Miki, Phil Jimenez; colors: Jeromy Cox; letters: VC's Cory Petit | Dexter Bennett, Betty Brant, Curt Connors, Carlie Cooper, Randall Crowne, Vin Gonzales, Bill Hollister, Lily Hollister, J. Jonah Jameson, Harry Osborn, Peter Parker, Spider-Man |
| 26 | - | cover reprint (on interior page) | 1 | pencils: Chris Bachalo; inks: Tim Townsend; colors: Chris Bachalo | Logan, Peter Parker, Spider-Man, Wolverine |
| 27 | Sometimes It Snows in April | comic story | 22 | script: Zeb Wells; pencils: Chris Bachalo; inks: Tim Townsend; colors: Studio F's Antonio Fabela, Chris Bachalo; letters: VC's Cory Petit | Dexter Bennett, Betty Brant, Logan, Peter Parker, Spider-Man, Doctor Strange, Stephen Strange, Wolverine, Wong |
| 28 | - | cover reprint (on interior page) | 1 | pencils: Chris Bachalo; inks: Tim Townsend; colors: Chris Bachalo | Peter Parker, Spider-Man |
| 29 | The Last Nameless Day | comic story | 22 | script: Zeb Wells; pencils: Chris Bachalo; inks: Tim Townsend; colors: Studio F's Antonio Fabela, Chris Bachalo; letters: VC's Cory Petit | Carlie Cooper, Vin Gonzales, J. Jonah Jameson, Peter Parker, Spider-Man |
| 30 | - | cover reprint (on interior page) | 1 | pencils: Chris Bachalo; inks: Tim Townsend; colors: Chris Bachalo | Carlie Cooper, Peter Parker, Spider-Man |
| 31 | Dead of Winter | comic story | 22 | script: Zeb Wells; pencils: Chris Bachalo; inks: Tim Townsend, Mark Irwin, Wayne Faucher, Jaime Mendoza, Al Vey; colors: Studio F's Antonio Fabela, Chris Bachalo; letters: VC's Cory Petit | Carlie Cooper, Vin Gonzales, Lily Hollister, Harry Osborn, Peter Parker, Spider-Man |
| 32 | - | cover reprint (on interior page) | 1 | pencils: Barry Kitson; inks: Mark Farmer; colors: Jeromy Cox | Lily Hollister, J. Jonah Jameson, Martin Li, Menace, Mister Negative, Harry Osborn, Peter Parker, Spider-Man |
| 33 | Freak the Third | comic story | 22 | script: Bob Gale; pencils: Barry Kitson; inks: Mark Farmer; colors: Avalon's Ian Hannin, Avalon's Matt Milla; letters: VC's Cory Petit | Curt Connors, Vin Gonzales, Bill Hollister, Lily Hollister, J. Jonah Jameson, Marla Madison Jameson, Menace, May Parker, Peter Parker, Spider-Man |
| 34 | - | cover reprint (on interior page) | 1 | pencils: Ed McGuinness; inks: Ed McGuinness; colors: Morry Hollowell | Peter Parker, Screwball, Spider-Man |
| 35 | Peter Parker, Paparazzi Part One: The Money Shot | comic story | 22 | script: Dan Slott; pencils: Marcos Martín; inks: Marcos Martín; colors: Javier Rodríguez; letters: VC's Cory Petit | Dexter Bennett, Piper Dali, Lily Hollister, J. Jonah Jameson, Harry Osborn, Paper Doll, Peter Parker, Robbie Robertson, Screwball, Spider-Man |
| 36 | - | cover reprint (on interior page) | 1 | script: Dan Slott; pencils: Marcos Martín; inks: Marcos Martín; colors: Marcos Martín | Piper Dali, Paper Doll, Peter Parker, Spider-Man |
| 37 | Peter Parker, Paparazzi: Flat Out Crazy | comic story | 22 | script: Dan Slott; pencils: Marcos Martín; inks: Marcos Martín; colors: Javier Rodríguez; letters: VC's Cory Petit | Dexter Bennett, Betty Brant, Carlie Cooper, Piper Dali, Gloria Grant, J. Jonah Jameson, Harry Osborn, Paper Doll, Peter Parker, Robbie Robertson, Spider-Man, Mary Jane Watson |
| 38 | Brand New Day | cover reprint (on interior page) | 1 | pencils: Marcos Martín; inks: Marcos Martín; colors: Marcos Martín | Piper Dali, Paper Doll, Peter Parker, Spider-Man |
| 39 | Peter Parker, Paparazzi: Photo Finished | comic story | 23 | script: Dan Slott; pencils: Marcos Martín; inks: Marcos Martín; colors: Javier Rodríguez; letters: VC's Cory Petit | Dexter Bennett, Betty Brant, Carlie Cooper, Piper Dali, Gloria Grant, Harry Osborn, Paper Doll, May Parker, Peter Parker, Robbie Robertson, Spider-Man, Mary Jane Watson |
| 40 | - | cover reprint (on interior page) | 1 | pencils: Mike McKone; inks: Andy Lanning; colors: Morry Hollowell; letters: Todd Klein | Peter Parker, Spider-Man |
| 41 | The Other Spider-Man | comic story | 22 | script: Bob Gale; pencils: Mike McKone; inks: Andy Lanning; colors: Jeromy Cox; letters: VC's Cory Petit | Morrie Bench, Bookie, Betty Brant, Jackson Brice, Daniel Brito, Donald Callahan, Coachwhip, Condor, Fancy Dan, Fer-de-Lance, Gordon Fraley, Vin Gonzales, Hydro-Man, Beatrix Keener |
| 42 | - | cover reprint (on interior page) | 1 | pencils: Mike McKone; inks: Marlo Alquiza; colors: Jeromy Cox; letters: Todd Klein | Jackson Brice, Daniel Brito, Fancy Dan, Montana, Peter Parker, Spider-Man |
| 43 | So Spider-Man Walks into a Bar and... | comic story | 22 | script: Bob Gale; pencils: Mike McKone; inks: Marlo Alquiza; colors: Jeromy Cox, Antonio Fabela; letters: VC's Cory Petit | Morrie Bench, Bookie, Donald Callahan, Coachwhip, Condor, Fer-de-Lance, Gordon Fraley, Hydro-Man, Beatrix Keener, Gustav Krueger, John Ladue, Martin Li, M'Gula, Mister Negative |
| 44 | - | cover reprint (on interior page) | 1 | pencils: Chris Bachalo; inks: Tim Townsend; colors: Chris Bachalo; letters: Todd Klein | James Beverley, Vin Gonzales, Martin Li, Mister Negative, Overdrive, Peter Parker, Spider-Man |
| 45 | Threeway Collision | comic story | 23 | script: Marc Guggenheim, Bob Gale, Dan Slott; pencils: Paulo Siqueira; inks: Amilton Santos, Paulo Siqueira; colors: Antonio Fabela; letters: VC's Cory Petit | Dexter Bennett, James Beverley, Carlie Cooper, Vin Gonzales, Martin Li, Mister Negative, Overdrive, Peter Parker, Spider-Man |
| 46 | - | cover reprint (on interior page) | 1 | pencils: Bryan Hitch; inks: Bryan Hitch; colors: Laura Martin | Peter Parker, Spider-Man |
| 47 | Amazing Spider-Man #546 Sketch Variant | cover reprint (on interior page) | 1 | pencils: Steve McNiven | Peter Parker, Spider-Man |
| 48 | - | cover reprint (on interior page) | 1 | pencils: David Finch; inks: David Finch; colors: Laura Martin | Peter Parker, Spider-Man |
| 49 | Amazing Spider-Man #549 Dynamic Forces Variant | cover reprint (on interior page) | 1 | pencils: John Romita Sr.; inks: John Romita Sr. | Lily Hollister, Menace, Peter Parker, Spider-Man |
| 50 | - | cover reprint (on interior page) | 1 | pencils: Adi Granov; inks: Adi Granov; colors: Adi Granov | Peter Parker, Spider-Man |
| 51 | - | cover reprint (on interior page) | 1 | pencils: Simone Bianchi; inks: Simone Bianchi; colors: Simone Peruzzi | Logan, Peter Parker, Spider-Man, Wolverine |
| 52 | Spider-Man: Swing Shift Corner Box Art | illustration | 1 | pencils: Steve McNiven; inks: Steve McNiven; colors: Steve McNiven | Peter Parker, Spider-Man |
| 53 | Free Comic Book Day 2007 Art | illustration | 3 | pencils: Phil Jimenez; inks: Andy Lanning; colors: Jeromy Cox | |
| 54 | Spider-Man Manifesto | text article | 5 | script: Tom Brevoort | |
| 55 | Top Secret Amazing Spider-Man 2008 Bible | character profile | 18 | script: Tom Brennan | Spider-Man; Aunt May Parker; Harry Osborn; Mary Jane Watson; J. Jonah Jameson; Dexter Bennett; Lily Hollister; Vin Gonzales; Carlie Cooper; Jackpot; Robbie Robertson; Betty Brant; Martin Li; Bill Hollister; Alan O'Neil; Menace; Freak; Mr. Negative; The Bookie; Screwball; Overdrive; Paper Doll |
| 56 | Amazing Spider-Man #564 | text story | 5 | script: Marc Guggenheim, Bob Gale, Dan Slott | |